The Influence of Starter Cultures on the Content of Vitamin B2 in Tea Fungus Beverages
Radomir V. Malbaa1, Milan Z. Maksimović2, Eva S. Lončar1, and Tatjana I. Branković2
1 Department for Analytical Chemistry, Faculty of Technology, University of Novi Sad, Novi Sad, Serbia and Montenegro
2 Institute of Hygiene, Military Medical Academy, Belgrade, Serbia and Montenegro
Corresponding author: Radomir V. Malbaa
	
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 CEJOEM 2004, Vol.10. No.1.: 79–83
Key words:
Tea fungus, kombucha, vitamins, fermented food, antioxidants, HPLC
Abstract:
The tea fungus beverage usually named kombucha belongs to a group of natural food products 
with no additives or potentially risky substances. Vitamins B2 and B6 are 
compounds that are produced during fermentation of the tea fungus. Beside their contribution to 
the nutritive value of the beverage, they are strong antioxidants. The task of this study was to 
investigate the production of vitamins B2 and B6 in fermentation performed 
by means of native kombucha and two starter cultures formed from native kombucha isolates that 
grew on sweetened black and green tea. The vitamins were quantified by HPLC method, which had not 
been common for this kind of samples. The highest vitamin B2 value of 
96 mg/L was noticed after 10 days of native kombucha 
fermentation (control series) on green tea substrate. Vitamin B6 content was less than 
30 mg/L in all cases.
Received: 1 August 2003
Accepted: 5 December 2003
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