Premature Chromosome Condensation (PCC) Assay in Biological Dosimetry

Gabriella Mészáros1, Doina Obreja2, Gabriella Bognár1, and György J. Köteles1

1 Frédéric Joliot-Curie National Research Institute for Radiobiology and Radiohygiene, József Fodor National Center of Public Health, Budapest, Hungary
2 Institute of Public Health, Bucharest, Romania

Corresponding author: Gabriella Mészáros
    Frédéric Joliot-Curie National Research Institute for Radiobiology and Radiohygiene
    József Fodor National Center of Public Health
    H-1221 Budapest
    Telephone: (+36) 1 482-2000
    Fax number: (+36) 1 482-2003
    E-mail: meszaros@hp.osski.hu

CEJOEM 2003, Vol.9. No.1.: 39–44

Key words:
PCC fragments, DNA damage, dose assessment, ionizing radiation

Abstract:
To investigate the efficacy of premature chromosome condensation (PCC) analysis for the assessment of radiation-induced damage, fresh human lymphocytes were irradiated with X- and gamma-rays of various doses and immediately fused with mitotic Chinese hamster ovary (CHO) cells in the presence of polyethylene-glycol (PEG). The shape of the obtained dose-response functions was linear and the values were nearly the same for each dose. Only slight interindividual differences were found. This technique seems to be appropriate as a complementary assay in biological dosimetry, especially at doses higher than 1 Gy.

Received: 28 July 2003
Accepted: 7 October 2003
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