**The Effect of Bryonia alba root Extracts on Exogenous and Endogenous Oxidative DNA Damage in Human Lymphocytes**

Armen K. Nersesyan

Cancer Research Center, Yerevan, Armenia and Rowett Research Institute, Aberdeen, UK

Corresponding author: Armen K. Nersesyan, Ph.D., D.Sc.
    Department of Genetics and Cytology,
    State University, 1 Alex Manoukian St,
    Yerevan 25, Armenia
    Telephone: +3741 552352
    Fax number: +3741 286922
    E-mail adress: genetik@ysu.am

CEJOEM 2001, Vol.7. Nos.3-4.:209-216

Key words:
Oxitative DNA damage, comet assay, “Loshtak” preparation, chemoprevention

Abbreviations:
LP = “Loshtak” preparation
AE = aqueous extract
ME = methanol extract
FPG = formamidopyrimidine glycolyse

Abstract:
The effect of aqueous and methanol extracts of “Loshtak” preparation (purified Bryonia alba roots) on exogenous and endogenous oxidative DNA damage was studied on human lymphocytes using the comet assay with endonuclease III and formamidopyrimidine DNA glycosylase. Extracts of Loshtak lack ability to protect human cells against exogenous DNA oxidative damage induced by H₂O₂ but protect them from the endogenous one. The ability of Loshtak preparation to protect human cells against endogenous oxidative DNA damage adds a new beneficial property to other effects (e. g., lack of genotoxic and carcinogenic activities, antimutagenic effect) of it.

Received: 11 February 2001
Accepted: 11 March 2001

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