Dose-Dependent Induction of Lipid Peroxidation and Oxidative Stress in Blood by Subchronic Lindane Exposure in Rats
Vandana Seth1, Rafat S. Ahmed1, Basu Dev Banerjee1, and
Illés Dési2
1 Department of Biochemistry, University College of Medical Sciences and G. T. B.
Hospital, University of Delhi, Shahdara, Delhi 110095, India
2 Department of Public Health and WHO Collaborating Center for Chemical Safety,
Faculty of General Medicine, Albert Szent-Györgyi Medical and Pharmaceutical Center, University of Szeged, Szeged, Hungary
Corresponding author: Dr. Basu Dev Banerjee
Department of Biochemistry,
University College of Medical Sciences and GTB Hospital, Shahdara
Delhi-110095, India
Fax number: Fax: (+91) 11 229 0495
E-mail: dbmi@ucms.ernet.in
CEJOEM 2000, Vol.6. No.4.:306-313
Key words:
Oxygen free radicals, glutathione, antioxidant enzymes, glutathione-S-transferase, glutathione reductase, pesticide, erythrocytes
Abstract:
Effect of subchronic oral administration of lindane (10, 30 and 50 mg/kg body weight per day for 30 days) was evaluated on lipid peroxidation, glutathione (GSH), and related enzymes and oxygen free radical (OFR) scavenging enzymes in blood samples obtained from male albino rats. A dose dependent increase in serum malondialdehyde level, activities of superoxide dismutase, catalase and glutathione peroxidase in erythrocytes and serum glutathione reductase and glutathione-S-transferase were found. However, GSH level in whole blood was significantly reduced. The present results suggest that OFRs scavenging enzymes in blood were elevated while combating oxidative stress and possible involvement of free radicals in lindane induced toxicity. Increased lipid peroxidation, coupled with altered level of GSH and OFR scavenging enzymes in blood, are discussed in the light of oxidative stress.
Received: 13 September 2000
Accepted: 05 December 2000
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