Time Dependency of Ni2+-Effect on in vitro Testosterone
Production by Mouse Leydig Cells
Zs. Forgács, K. Paksy, P. Lázár
National Institute of Occupational Health, Budapest, Hungary
CEJOEM 1997, Vol.3. No.4.:334
Earlier, Ni2+ has been reported to decrease hCG-supported testosterone
(T) production by Leydig cells cultured for
48 h in a dose dependent manner. It was stated that compromising effect
of Ni2+ can be ameliorated by addition of an equimolar or twice
the equimolar concentrations of histidine (His) or cystein (Cys).
In the present study stimulated T production by
Leydig cell incubated for various times in the presence of 1,000 µM
Ni2+ was measured. In order to be able to observe a treatment
related effect Ni2+-exposure had to be maintained at least for
4 hours. Complete protection against Ni2+ in the presence of 2,000 µM
His occurred in cells incubated for
16 h, whereas Cys protected for 8 h only.
The time duration within which the Ni2+
complex amino acid has to be added to the culture was also determined.
Ni-induced alteration in hormone production was only moderately affected
in case His or Cys was added to the system within 4 h, addition of amino
acid later than that failed to protect the effect of Ni2+.
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